Characteristics of Root Cells during In Vitro Rhizogenesis under Action of NaCl in Two Tomato Genotypes Differing in Salt Tolerance

Understanding the mechanisms of plant salt tolerance as a complex trait is an integral part many studies, results which have been used in breeding process. The aim this study was to compare root response two tomato (Solanum lycopersicum L.) genotypes (breeding line YaLF and cultivar Recordsmen) differing tolerance. Rhizogenesis induced shoots vitro with different concentrations NaCl culture medium. A number morphobiological cytological parameters were evaluated at organ, tissue, cellular levels for possible use comprehensive assessment influence caused disruption cell cycle redistribution cells phases cycle. An increase degree vacuolization shown cv Recordsmen 75 150 mM NaCl. Under action, increase/decrease length such columella (both genotypes) epidermal (in NaCl) shown. Differences between demonstrated by changes area central cylinder primary cortex cells, well Snucleolus/Snucleus ratio these cells. Transmission electron microscopy (TEM) showed modification chromatin structure genotypes. Various cytoskeletal disorders revealed interphase immunofluorescent staining under saline conditions. These morphometric can be comparative evaluation varieties.